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ImmunohistochemistryAntibody data
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- Product number
- PA5-42572 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCTP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: EVTVYDEDRD RSADFLGKVA IPLLSIQNGE QKAYVLKNKQ LTGPTKGVIY Sequence homology: Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 100%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references The DNA methylation-regulated MCTP1 activates the drug-resistance of esophageal cancer cells.
Kong L, Yang W, Chen L, Qian L
Aging 2021 Feb 11;13(3):3342-3352
Aging 2021 Feb 11;13(3):3342-3352
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human fetal muscle cells using an anti-MCTP1 polyclonal antibody (Product # PA5-42572).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human adult placenta cells using an anti-MCTP1 polyclonal antibody (Product # PA5-42572).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Jurkat cell lysate using an anti-MCTP1 polyclonal antibody (Product # PA5-42572).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Effects of a forced reversal of the MCTP1 levels on the drug resistance of Kyse450 cells. The levels of MCTP1 mRNA ( A and B ) and protein level ( C ) determined by qRT-PCR and western blot analysis in the nine esophageal cancer cell lines. The levels of mRNA ( D ) and MCTP1 protein level ( E ) determined by qRT-PCR and western blot analysis in the si-MCTP1-transfected versus the NC-transfected Kyse450 cells. ( F ) The CCK8 assays showing cell death triggered by an IC 50 dose of drug in Kyse510 cells transfected with three different regions' si-MCTP1 versus the negative control (NC) assayed 72hr after treatment with the IC 50 dose of drugs.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Effects of a forced reversal of the MCTP1 levels on the drug resistance of Kyse510 cells. ( A ) MCTP1 overexpression lentivirus vector map. ( B ) Representative areas of light and fluorescent of Kyse510 cells infected with MCTP1 overexpression lentivirus vector and the negative control (NC). MCTP1 mRNA ( C ) and protein ( D ) level determined by qRT-PCR and western blot analysis of Kyse510 cells infected with MCTP1 overexpression lentivirus vector and the negative control (NC). ( E ) The cell death triggered by an IC 50 dose of four drugs in Kyse510 cells infected with MCTP1 overexpression lentivirus vector versus the negative control (NC) assayed 72hr after treatment with the IC 50 dose of drugs.
