600-401-C52S

antibody from Invitrogen Antibodies

Targeting: HAUS8 HICE1, MGC20533, NY-SAR-48

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

600-401-C52S

Provider

Invitrogen Antibodies

Product name

Phospho-HICE1 pS70 (Ser70) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

1.22 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of Rabbit Anti-Hice1 pS70 antibody. Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant (70A). Load: 35 µg per lane. Primary antibody: Hice1 pS70 antibody at 0.5 µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 44.8 kDa, 48 kDa for Hice1 pS70. Other band(s): none.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of Rabbit anti-HICE1 pS70 antibody. Lane 1: 293T Null. Lane 2: 293T WT Hice1. Lane 3: 293T S70A Hice1. Load: 14 µl per lane. Primary antibody: HICE1 pS70 antibody at 0.75 µg/mL overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 48 kDa, 48 kDa for HICE1 pS70. Other band(s): HICE1 pS70 splice variants and isoforms.