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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP1-77746 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77746, RRID:AB_11039642
- Product name
- Rabbit Polyclonal HAUS8 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody reacts with endogenous Hice1 protein. A BLAST analysis was used to suggest reactivity with Hice1 from human based on a 100% homology.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: HAUS8 Antibody [NBP1-77746] - Analysis shows detection of a 45 kDa band corresponding to endogenous HICE1 in lysates of S phase HeLa cells silenced for either control Luciferase or HAUS8. In right lane (HAUS8): lysates from sh-HAUS8 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-HICE1 Antibody was used at 1:10,000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection.
