GTX48802

antibody from GeneTex

Targeting: VAMP3 CEB

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX48802

Provider

GeneTex

Proper citation

GeneTex Cat#GTX48802, RRID:AB_11165518

Product name

Cellubrevin antibody [k2A2]

Antibody type

Monoclonal

Host

Mouse

Vial size

100 µl

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot showing detection of 0.1 µg of recombinant DbpB protein. Lane 1: Molecular weight markers. Lane 2: MBP-DbpB fusion protein (arrow; expected MW = 60.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).

Validation comment

WB