MA1-840

antibody from Invitrogen Antibodies

Targeting: NCOA1 bHLHe74, F-SRC-1, KAT13A, NCoA-1, RIP160, SRC1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-840

Provider

Invitrogen Antibodies

Product name

SRC1 Monoclonal Antibody (1135/H4)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA1-840 detects steroid receptor coactivator-1 (SRC-1) from mouse and primate tissues and cells as well as recombinant human SRC-1 protein. MA1-840 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot MA1-840 detects an ~165 kDa protein representing SRC-1 in COS cell extract. Also by Western blot, MA1-840 detects an unidentified protein at ~30-40 kDa. This antibody does not react with NIH-3T3 cells in Western blot. The MA1-840 immunogen is a synthetic peptide corresponding to residues 477-947 of human SRC-1. The epitope for MA1-840 has been mapped to the extreme C-terminal end of this fragment.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

1135/H4

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Nuclear receptor corepressors Ncor1 and Ncor2 (Smrt) are required for retinoic acid-dependent repression of Fgf8 during somitogenesis.

Kumar S, Cunningham TJ, Duester G
Developmental biology 2016 Oct 1;418(1):204-215

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SIP1/NHERF2 enhances estrogen receptor alpha transactivation in breast cancer cells.

Meneses-Morales I, Tecalco-Cruz AC, Barrios-García T, Gómez-Romero V, Trujillo-González I, Reyes-Carmona S, García-Zepeda E, Méndez-Enríquez E, Cervantes-Roldán R, Pérez-Sánchez V, Recillas-Targa F, Mohar-Betancourt A, León-Del-Río A
Nucleic acids research 2014 Jun;42(11):6885-900

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D538G mutation in estrogen receptor-α: A novel mechanism for acquired endocrine resistance in breast cancer.

Merenbakh-Lamin K, Ben-Baruch N, Yeheskel A, Dvir A, Soussan-Gutman L, Jeselsohn R, Yelensky R, Brown M, Miller VA, Sarid D, Rizel S, Klein B, Rubinek T, Wolf I
Cancer research 2013 Dec 1;73(23):6856-64

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Expression patterns of the steroid receptor coactivator family in human ovarian endometriosis.

Kumagami A, Ito A, Yoshida-Komiya H, Fujimori K, Sato A
The journal of obstetrics and gynaecology research 2011 Oct;37(10):1269-76

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Constitutive activation of the mitogen-activated protein kinase pathway impairs vitamin D signaling in human prostate epithelial cells.

Zhang Z, Kovalenko P, Cui M, Desmet M, Clinton SK, Fleet JC
Journal of cellular physiology 2010 Aug;224(2):433-42

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Role of steroid receptor coactivators in glucocorticoid and transforming growth factor beta regulation of plasminogen activator inhibitor gene expression.

Li G, Heaton JH, Gelehrter TD
Molecular endocrinology (Baltimore, Md.) 2006 May;20(5):1025-34

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Role of SRC-1 in the promotion of prostate cancer cell growth and tumor progression.

Agoulnik IU, Vaid A, Bingman WE 3rd, Erdeme H, Frolov A, Smith CL, Ayala G, Ittmann MM, Weigel NL
Cancer research 2005 Sep 1;65(17):7959-67

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Role of CBP in regulating HIF-1-mediated activation of transcription.

Ruas JL, Poellinger L, Pereira T
Journal of cell science 2005 Jan 15;118(Pt 2):301-11

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Nuclear coactivator-62 kDa/Ski-interacting protein is a nuclear matrix-associated coactivator that may couple vitamin D receptor-mediated transcription and RNA splicing.

Zhang C, Dowd DR, Staal A, Gu C, Lian JB, van Wijnen AJ, Stein GS, MacDonald PN
The Journal of biological chemistry 2003 Sep 12;278(37):35325-36

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Steroid receptor coactivator expression throughout the menstrual cycle in normal and abnormal endometrium.

Gregory CW, Wilson EM, Apparao KB, Lininger RA, Meyer WR, Kowalik A, Fritz MA, Lessey BA
The Journal of clinical endocrinology and metabolism 2002 Jun;87(6):2960-6

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Ordered recruitment of histone acetyltransferases and the TRAP/Mediator complex to thyroid hormone-responsive promoters in vivo.

Sharma D, Fondell JD
Proceedings of the National Academy of Sciences of the United States of America 2002 Jun 11;99(12):7934-9

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Role of CBP/p300 and SRC-1 in transcriptional regulation of the pulmonary surfactant protein-A (SP-A) gene by thyroid transcription factor-1 (TTF-1).

Yi M, Tong GX, Murry B, Mendelson CR
The Journal of biological chemistry 2002 Jan 25;277(4):2997-3005

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Attenuation of glucocorticoid signaling through targeted degradation of p300 via the 26S proteasome pathway.

Li Q, Su A, Chen J, Lefebvre YA, Haché RJ
Molecular endocrinology (Baltimore, Md.) 2002 Dec;16(12):2819-27

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Endogenously expressed estrogen receptor and coactivator AIB1 interact in MCF-7 human breast cancer cells.

Tikkanen MK, Carter DJ, Harris AM, Le HM, Azorsa DO, Meltzer PS, Murdoch FE
Proceedings of the National Academy of Sciences of the United States of America 2000 Nov 7;97(23):12536-40

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Recruitment of nuclear receptor corepressor and coactivator to the retinoic acid receptor by retinoid ligands. Influence of DNA-heterodimer interactions.

Klein ES, Wang JW, Khalifa B, Gavigan SA, Chandraratna RA
The Journal of biological chemistry 2000 Jun 23;275(25):19401-8

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Temporal formation of distinct thyroid hormone receptor coactivator complexes in HeLa cells.

Sharma D, Fondell JD
Molecular endocrinology (Baltimore, Md.) 2000 Dec;14(12):2001-9

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A steroid receptor coactivator, SRA, functions as an RNA and is present in an SRC-1 complex.

Lanz RB, McKenna NJ, Onate SA, Albrecht U, Wong J, Tsai SY, Tsai MJ, O'Malley BW
Cell 1999 Apr 2;97(1):17-27

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Partial hormone resistance in mice with disruption of the steroid receptor coactivator-1 (SRC-1) gene.

Xu J, Qiu Y, DeMayo FJ, Tsai SY, Tsai MJ, O'Malley BW
Science (New York, N.Y.) 1998 Mar 20;279(5358):1922-5

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Partial hormone resistance in mice with disruption of the steroid receptor coactivator-1 (SRC-1) gene.

Xu J, Qiu Y, DeMayo FJ, Tsai SY, Tsai MJ, O'Malley BW
Science (New York, N.Y.) 1998 Mar 20;279(5358):1922-5

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Steroid receptor coactivator-1 is a histone acetyltransferase.

Spencer TE, Jenster G, Burcin MM, Allis CD, Zhou J, Mizzen CA, McKenna NJ, Onate SA, Tsai SY, Tsai MJ, O'Malley BW
Nature 1997 Sep 11;389(6647):194-8

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot detection of SRC-1 on COS cells using Product # MA1-840.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SRC1 was performed by loading 25 µg of LNCaP (lane 1), PC-3 (lane 2) and NIH-3T3 (lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a SRC1 monoclonal antibody (Product # MA1-840) at a dilution of 1:2000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~152 and ~157 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4. NHERF2 and SRC-1 form a complex to enhance ERalpha transactivation. ( A ) NHERF2 enhances the activity of the steroid receptor coactivator 1 (SRC-1). Control MCF7 cells and FLAG-NHERF2-MCF7 cells were transfected with pcDNA-SRC1 vector and ERalpha activity was determined by luciferase assay using the ERE-TK-Luc reporter. The cells were grown in the absence (white bars) or presence (gray bars) of E2 for 48 h. All results were normalized to luciferase activity observed in control MCF7 cells transfected with empty pcDNA vector ('Control'). Results were expressed as mean relative luciferase activity +- S.E. ( B ) NHERF2 knockdown impairs SRC1 coactivator activity on ERalpha transactivation. MCF7 cells were transfected with pcDNA-SRC1 in the presence of an NHERF2-specific siRNA (gray bar) or an unrelated control siRNA (white bars) as described in Materials and Methods. ERalpha activity was determined using the ERE-luciferase reporter in the presence of 100 nM E2. The results were normalized to luciferase activity of control MCF7 cells transfected with empty pcDNA vector and the unrelated control siRNA. ( C ) NHERF2 interacts with ERalpha and SRC-1 in ZR-75-1 breast cancer cells. Whole cell protein extracts were subjected to IP using anti-ERalpha (upper panel), anti-SRC-1 (lower panel) followed by immunoblot using anti-NHERF2, anti-ER or anti-SRC-1 antibodies. As a negative control immunoprecipitation was performed using anti-IgG (IgG, upper and lower panels). (