PA5-22174

antibody from Invitrogen Antibodies

Targeting: UBQLN1 DA41, DSK2, PLIC-1, XDRP1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22174

Provider

Invitrogen Antibodies

Product name

Ubiquilin 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (90%), Rat (90%), Sheep (96%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

p62/Sequestosome 1 levels increase and phosphorylation is altered in Cx50D47A lenses, but deletion of p62/sequestosome 1 does not improve transparency.

Jara O, Mysliwiec H, Minogue PJ, Berthoud VM, Beyer EC
Molecular vision 2020;26:204-215

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Ubiquilin-1 in paraformaldehyde-fixed A549 cells using a Ubiquilin-1 polyclonal antibody (Product # PA5-22174) at a 1:200 dilution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 7 Levels of ubiquilins are altered in lenses of homozygous Cx50D47A mice with or without the deletion of p62. A - C : Immunoblots show levels of UBQLN1 ( A ), UBQLN2 ( B ), and UBQLN4 ( C ) in lens homogenates prepared from 1-month-old wild-type ( p62 +/+ -Cx50 +/+ ), or heterozygous ( p62 +/+ -Cx50 +/D47A ) or homozygous ( p62 +/+ -Cx50 D47A/D47A ) Cx50D47A mice. The migration positions of the molecular mass markers are indicated on the left. The graphs show the densitometric values of the immunoreactive ubiquilin bands. Data are presented as the mean (bar) + standard error of the mean (SEM; n = 3 sets) in arbitrary units (a.u.). D - F : Immunoblots show levels of UBQLN1 ( D ), UBQLN2 ( E ), and UBQLN4 ( F ) in lens homogenates prepared from 1-month-old homozygous Cx50D47A mice that were wild-type ( p62 +/+ -Cx50 D47A/D47A ) or heterozygous ( p62 +/- -Cx50 D47A/D47A ) or homozygous ( p62 -/- -Cx50 D47A/D47A ) p62 . The migration positions of the molecular mass markers are indicated on the left. The graphs show the densitometric values of the immunoreactive ubiquilin bands. Data are presented as the mean (bar) + SEM (n = 3 sets) in arbitrary units (a.u.). Asterisks denote statistically significant differences from wild-type lenses (p