PA5-96312

antibody from Invitrogen Antibodies

Targeting: HUS1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96312

Provider

Invitrogen Antibodies

Product name

HUS1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MKFRAKIVDG ACLNHFTRIS NMIAKLAKTC TLRISPDKLN FILCDKLANG GVSMWCELEQ ENFFNEFQME GVSAENNEIY LELTSENLSR ALKTAQNARA LKIKLTNKHF PCLTVSVELL SMSSSSRIVT HDIPIKVIPR KLWKDLQEPV VPDPDVSIYL PVLKTMKSVV EKMKNISNHL VIEANLDGEL NLKIETELVC VTTHFKDLGN PPLASESTHE DRNVEHMAEV HIDIRKLLQF LAGQQVNPTK ALCNIVNNKM VHFDLLHEDV SLQYFIPALS; Positive Samples: OVCAR-3, K-562, 293T; Cellular Location: Cytoplasm, Nucleus

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.71 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HUS1 was performed in U2OS cells using HUS1 Polyclonal Antibody (Product # PA5-96312). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HUS1 was performed in U2OS cells using HUS1 Polyclonal Antibody (Product # PA5-96312). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HUS1 in U2OS cells. Samples were incubated with HUS1 Polyclonal antibody (Product # PA5-96312). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HUS1 in U2OS cells. Samples were incubated with HUS1 Polyclonal antibody (Product # PA5-96312). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of HUS1 in 200 μg extracts of 293T cells. Samples were precipitated with 1 μg HUS1 Polyclonal antibody (Product # PA5-96312). Western blot was performed from the immunoprecipitate using HUS1 Polyclonal antibody (Product # PA5-96312) at a dilution of 1:1,000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of HUS1 was performed in 200 µg extracts of 293T cells using HUS1 Polyclonal Antibody (Product # PA5-96312). Western blot was performed from the immunoprecipitate using HUS1 Polyclonal Antibody at a dilution of 1:1000.