PA5-96623

antibody from Invitrogen Antibodies

Targeting: DCD AIDD, DCD-1, DSEP, HCAP, PIF

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96623

Provider

Invitrogen Antibodies

Product name

DCD Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: YDPEAASAPG SGNPCHEASA AQKENAGEDP GLARQAPKPR KQRSSLLEKG LDGAKKAVGG LGKLGKDAVE DLESVGKGAV HDVKDVLDSV L; Positive Samples: Mouse skin

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.7 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DCD in HeLa cells. Samples were incubated with DCD Polyclonal antibody (Product # PA5-96623) using a dilution of 1:8,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DCD in 293T cells. Samples were incubated with DCD Polyclonal antibody (Product # PA5-96623) using a dilution of 1:8,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of DCD was performed in MCF-7 cells using DCD Polyclonal Antibody (Product # PA5-96623).