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- Antibody Data
- Antigen structure
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- Western blot [1]
- Immunohistochemistry [5]
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- Product number
- RQ6902 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Sigma1-receptor Antibody / SIGMAR1
- Antibody type
- Polyclonal
- Description
- This highly specific Sigma1-receptor antibody is suitable for use in Western blot/Immunohistochemistry/Direct ELISA applications with human, mouse and rat, monkey samples.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Sigma1-receptor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human Caco-2, 3) human 293T, 4) human HepG2, 5) human A549, 6) human COLO-320, 7) human U-87 MG, 8) monkey COS-7, 9) mouse liver and mouse C2C12 cell lysate with Sigma1-receptor antibody. Predicted molecular weight ~25 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human stomach cancer tissue with Sigma1-receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human adenocarcinoma of the right colon with Sigma1-receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver cancer with Sigma1-receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse colon tissue with Sigma1-receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat colon tissue with Sigma1-receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
