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LearnPA5-18901
antibody from Invitrogen Antibodies
Targeting: PNPLA3
adiponutrin, ADPN, C22orf20, dJ796I17.1, FLJ22012, iPLA2epsilon
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-18901 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PNPLA3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with canine, porcine and rat based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Binge alcohol alters PNPLA3 levels in liver through epigenetic mechanism involving histone H3 acetylation.
Restrepo RJ, Lim RW, Korthuis RJ, Shukla SD
Alcohol (Fayetteville, N.Y.) 2017 May;60:77-82
Alcohol (Fayetteville, N.Y.) 2017 May;60:77-82
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Mouse Heart lysate using Product # PA5-18901 at a concentration of 0.05 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PNPLA3 by a PNPLA3 monoclonal antibody (Product # PA5-18901) at a concentration of 0.01 µg/mL. Mouse (A) and Rat (B) Adrenal gland lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PNPLA3/Adiponutrin in Mouse (A) and Rat (B) Adrenal gland lysate (35µg protein in RIPA buffer). Samples were probed with the PNPLA3/Adiponutrin antibody (Product # PA5-18901, 0.01µg/mL) for 1 hour. Western blot was detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Mouse Heart lysate using Product # PA5-18901 at a concentration of 0.05 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PNPLA3/Adiponutrin in paraffin embedded Human Skin tissue. Steamed antigen retrieval was performed with citrate buffer pH 6, followed by application of the PNPLA3/Adiponutrin antibody (Product # PA5-18901) (3.8µg/mL) and AP-staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
