Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-30684 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDAH2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, MCF-7. Predicted reactivity: Mouse (95%), Rat (95%), Rhesus Monkey (99%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DDAH2 Polyclonal Antibody (Product # PA5-30684). Sample (30 µg of whole cell lysate). Lane A: MCF-7. 12% SDS PAGE. DDAH2 Polyclonal Antibody (Product # PA5-30684) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DDAH2 Polyclonal Antibody detects DDAH2 protein at mitochondria by immunofluorescent analysis. Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 15 min. Green: DDAH2 protein stained by DDAH2 Polyclonal Antibody (Product # PA5-30684) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DDAH2 Polyclonal Antibody detects DDAH2 protein at mitochondria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. DDAH2 Polyclonal Antibody (Product # PA5-30684) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.