HPA034537

antibody from Atlas Antibodies

Targeting: SAMM50 CGI-51, OMP85, SAM50, TOB55, TRG-3, YNL026W

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA034537

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA034537, RRID:AB_10602794

Product name

Anti-SAMM50

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

HARSLEPLPSSGPDFGGLGEEAEFVEVEPEAKQEI
LENKDVVVQHVHFDGLGRTKDDIIICEIGDVFKAK
NLIEVMRKSHEAREKLLRLGIFRQVDVLID


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

Identification of Human N-Myristoylated Proteins from Human Complementary DNA Resources by Cell-Free and Cellular Metabolic Labeling Analyses.

Takamitsu E, Otsuka M, Haebara T, Yano M, Matsuzaki K, Kobuchi H, Moriya K, Utsumi T
PloS one 2015;10(8):e0136360

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human kidney and pancreas tissues using HPA034537 antibody. Corresponding SAMM50 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human parathyroid gland shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human parathyroid gland shows very strong granular cytoplasmic positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human small intestine shows strong cytoplasmic granular positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows strong cytoplasmic granular positivity in Leydig cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows very strong cytoplasmic granular positivity in cells in tubules.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows moderate cytoplasmic granular positivity in exocrine glandular cells.

Sample type

HUMAN