NBP1-84509

antibody from Novus Biologicals

Targeting: SAMM50 CGI-51, OMP85, SAM50, TOB55, TRG-3, YNL026W

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-84509

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-84509, RRID:AB_11022678

Product name

Rabbit Polyclonal SAMM50 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human SAMM50 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Identification of Human N-Myristoylated Proteins from Human Complementary DNA Resources by Cell-Free and Cellular Metabolic Labeling Analyses.

Takamitsu E, Otsuka M, Haebara T, Yano M, Matsuzaki K, Kobuchi H, Moriya K, Utsumi T
PloS one 2015;10(8):e0136360

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: SAMM50 Antibody [NBP1-84509] - Simple Western lane view shows a specific band for SAMM50 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: SAMM50 Antibody [NBP1-84509] - Electropherogram image(s) of corresponding Simple Western lane view. SAMM50 antibody was used at 1:4 dilution on RT-4 and U-251MG lysate(s).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SAMM50 Antibody [NBP1-84509] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SAMM50 Antibody [NBP1-84509] - Analysis in human cell line HL-60.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human pancreas shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human parathyroid gland shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining in human parathyroid gland and pancreas tissues using anti-SAMM50 antibody. Corresponding SAMM50 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human testis shows strong cytoplasmic granular positivity in Leydig cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human pancreas shows moderate cytoplasmic granular positivity in exocrine glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human kidney shows very strong cytoplasmic granular positivity in cells in tubules.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Analysis in human kidney and pancreas tissues. Corresponding SAMM50 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human small intestine shows strong cytoplasmic granular positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human parathyroid gland shows very strong granular cytoplasmic positivity in glandular cells.