H00000239-M01

antibody from Abnova Corporation

Targeting: ALOX12 12S-LOX

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000239-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000239-M01, RRID:AB_1111664

Product name

ALOX12 monoclonal antibody (M01), clone 2D10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant ALOX12.

Antigen sequence

PPPTTKEDVTMATVMGSLPDVRQACLQMAISWHLS
RRQPDMVPLGHHKEKYFSGPKPKAVLNQFRTDLEK
LEKEITARNEQLDWPYEYLKPSCIENSVTI


Isotype

IgG

Antibody clone number

2D10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Systematic analysis of rat 12/15-lipoxygenase enzymes reveals critical role for spinal eLOX3 hepoxilin synthase activity in inflammatory hyperalgesia.

Gregus AM, Dumlao DS, Wei SC, Norris PC, Catella LC, Meyerstein FG, Buczynski MW, Steinauer JJ, Fitzsimmons BL, Yaksh TL, Dennis EA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2013 May;27(5):1939-49

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ALOX12 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol