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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [5]
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Validation data
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- Product number
- LS-C340333 - Provider product page
- Provider
- LSBio
- Product name
- WDR61 Antibody (aa92-305, clone OTI4C4) LS-C340333
- Antibody type
- Monoclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI4C4
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WDR61 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WDR61.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of extracts (10ug) from 5 different cell lines and 4 human tissue by using anti-WDR61 monoclonal antibody (1: HeLa; 2: HepG2; 3: K562; 4: Mcf7; 5: SVT2; 6: Liver; 7: Testis; 8: Brain; 9: Uterus)at 1:200 dilution.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-WDR61 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Human prostate tissue using anti-WDR61 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-WDR61 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Human prostate tissue using anti-WDR61 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-WDR61 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
