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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AP14750PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP14750PU-N, RRID:AB_1748318
- Product name
- anti ALOX15 / LOG15 (C-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 619~649 amino acids from the C-terminal region of Human ALOX15.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of anti-ALOX15 Antibody (C-term) in Ramos cell line lysates (35ug/lane).ALOX15(arrow) was detected using the purified Pab.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- ALOX15 Antibody (C-term) (AP14750PU-N) western blot analysis in Hela (Lane 1), A549 (Lane 2), K562 (Lane 3), MDA-MB435 (Lane 4) cell line lysates (35ug/lane).This demonstrates the ALOX15 antibody detected the ALOX15 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of ALOX15 Antibody (C-term) (AP14750PU-N) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lymph tissue reacted with ALOX15 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
