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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- MA5-25853 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALOX15 Monoclonal Antibody (OTI3G8)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI3G8
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Growth State-Dependent Expression of Arachidonate Lipoxygenases in the Human Endothelial Cell Line EA.hy926.
Sabbir MG, Wigle JT, Taylor CG, Zahradka P
Cells 2022 Aug 10;11(16)
Cells 2022 Aug 10;11(16)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALOX15 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with ALOX15 (Product # MA5-25853) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALOX15 in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells using 35 µg per lane. Samples were probed with ALOX15 (Product # MA5-25853) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALOX15 in 3T3 cells, mouse brain, mouse liver, mouse spleen lysate using 10 µg per lane. Samples were probed with ALOX15 (Product # MA5-25853) monoclonal antibody at a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ALOX15 in COS7 cells. Cells were transfected with a plasmid overexpressing ALOX15 and probed with a ALOX15 monoclonal antibody (Product # MA5-25853).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoblot analysis of ALOX15. ( A ): Immunoblots of ALOX15 and GAPDH (loading control) in confluent EA.hy926 cells. Three replicates are shown. M, molecular mass markers. ( B ): Immunoblots of ALOX15 and GAPDH (loading control) in MDA MB231 (MDA-MB) and MCF7 cells. Three replicates are shown for each cell-type. ( C ): Immunoblots of ALOX15 (upper blot) and Flag (lower blot) in HEK293 transiently transfected with Flag-tagged ALOX15 expression plasmid (ALOX15) or empty plasmid (Empty), as indicated. Molecular mass markers were used to determine the relative size of the bands. Data are representative of three separate experiments.
