M01192

antibody from Boster Biological Technology

Targeting: CHAT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M01192

Provider

Boster Biological Technology

Product name

Anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for Choline Acetyltransferase/CHAT detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, ICC, WB. Reactive species: Human, Mouse, Rat Choline Acetyltransferase/CHAT information: Molecular Weight: 82536 MW; .

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

ABBO-3

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

Catalpol regulates cholinergic nerve system function through effect on choline acetyl-transferase not M receptor affinity.

Wang JH, Xie H, Zhao TK, Kang B
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2015 Feb;69:291-6

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of CHAT expression in SH-SY5Y cell lysate (M01192). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHAT monoclonal antibody (Catalog # M01192) overnight at 4

Additional image