20747-1-AP

antibody from Proteintech Group

Targeting: CHAT

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

20747-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#20747-1-AP, RRID:AB_10898169

Product name

CHAT antibody

Antibody type

Polyclonal

Description

CHAT antibody (Cat. #20747-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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Enhanced Contractive Tension and Upregulated Muscarinic Receptor 2/3 in Colorectum Contribute to Constipation in 6-Hydroxydopamine-Induced Parkinson's Disease Rats.

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Frontiers in aging neuroscience 2021;13:770841

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Activation of α7nAChR Protects Against Gastric Inflammation and Dysmotility in Parkinson's Disease Rats.

Zhou L, Zheng LF, Zhang XL, Wang ZY, Yao YS, Xiu XL, Liu CZ, Zhang Y, Feng XY, Zhu JX
Frontiers in pharmacology 2021;12:793374

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Dopamine receptor 2 downregulation and brain-derived neurotrophic factor upregulation in the paraventricular nucleus are correlated with brown adipose tissue thermogenesis in rats with bilateral substantia nigra lesions.

Zhang Y, Zhou L, Lian H, Zhang Y, Tong S, Wang Z
Journal of chemical neuroanatomy 2021 Nov;117:102016

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BDNF-overexpressing human umbilical cord mesenchymal stem cell-derived motor neurons improve motor function and prolong survival in amyotrophic lateral sclerosis mice.

Wang J, Hu W, Feng Z, Feng M
Neurological research 2021 Mar;43(3):199-209

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Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.

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Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine.

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Stem cells translational medicine 2021 Jun;10(6):922-938

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Synergistic Effect of the Long-Term Overexpression of Bcl-2 and BDNF Lentiviral in Cell Protecting against Death and Generating TH Positive and CHAT Positive Cells from MSC.

Borkowska P, Zielinska A, Paul-Samojedny M, Stojko R, Kowalski J
International journal of molecular sciences 2021 Jun 30;22(13)

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Mixture of Phlorotannin and Fucoidan from Ecklonia cava Prevents the Aβ-Induced Cognitive Decline with Mitochondrial and Cholinergic Activation.

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Electroacupuncture improves cardiac function and reduces infarct size by modulating cardiac autonomic remodeling in a mouse model of myocardial ischemia.

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Journal of neuropathology and experimental neurology 2017 Feb 1;76(2):70-88

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Intestinal injury in the rat model of 17α-ethynylestradiol-induced intrahepatic cholestasis.

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Journal of digestive diseases 2016 Nov;17(11):756-763

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Myocardial Infarction Causes Transient Cholinergic Transdifferentiation of Cardiac Sympathetic Nerves via gp130.

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The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Jan 13;36(2):479-88

---

Alleviating effects of Bushen-Yizhi formula on ibotenic acid-induced cholinergic impairments in rat.

Hou XQ, Zhang L, Yang C, Rong CP, He WQ, Zhang CX, Li S, Su RY, Chang X, Qin JH, Chen YB, Xian SX, Wang Q
Rejuvenation research 2015 Apr;18(2):111-27

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 20747-1-AP(CHAT antibody) at dilution of 1:500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using CHAT antibody 20747-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human stomach using 20747-1-AP(CHAT antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human stomach using 20747-1-AP(CHAT antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue