PA5-29653

antibody from Invitrogen Antibodies

Targeting: CHAT

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29653

Provider

Invitrogen Antibodies

Product name

ChAT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: IMR32, mouse brain, rat brain, DDDDK-tagged CHAT-transfected 293T. Predicted reactivity: Mouse (93%), Rat (92%), Pig (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.23 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Focal impaired awareness seizures in a rodent model: A functional anatomy.

Adotevi N, Kapur J
Epilepsia open 2022 Mar;7(1):110-123

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Targeting the tamoxifen receptor within sodium channels to block osteoarthritic pain.

McCollum MM, Larmore M, Ishihara S, Ng LCT, Kimura LF, Guadarrama E, Ta MC, Vien TN, Frost GB, Scheidt KA, Miller RE, DeCaen PG
Cell reports 2022 Aug 23;40(8):111248

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Angiopoietin-1 Knockout Mice as a Genetic Model of Open-Angle Glaucoma.

Thomson BR, Grannonico M, Liu F, Liu M, Mendapara P, Xu Y, Liu X, Quaggin SE
Translational vision science & technology 2020 Mar;9(4):16

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ChAT was performed in DIV14 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: ChAT Polyclonal Antibody (Product # PA5-29653) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 3 Multiple cortical structures are activated during a focal impaired awareness seizure. (A) Coronal sections at bregma 0.13 mm showing TRAPed neurons in the anterior cingulate cortex during a seizure (top panel) and in controls (bottom panel) in merged images showing NeuN immunolabels in green and TdT + cells in red. (B) Coronal sections at bregma -1.91 mm showing TRAPed neurons in the retrosplenial and parietal cortices during a seizure (top panel) and in controls (bottom panel). (C) Horizontal sections at bregma -1.68 mm showing TdT activation in the frontal, orbital, motor, somatosensory, and visual cortices with (left panel) or without (right panel) a seizure. Merged images show TdT + cells (red) and ChAT immunolabeling (green). (D) Coronal sections at bregma 1.09 mm showing TRAPed neurons in the piriform (layers 1, 2, and 3) and endopiriform cortex. (E) A greater number of TdT-labeled cells were present in the ACC during a behavioral grade 2 seizure compared with controls (control 166 +- 10, seizure 274 +- 26; n = 5 mice per group, t( 5.22) = 5.65, P = .002). (F) More piriform cortex cell body layer II neurons were TdT-labeled compared with controls (control 100 +- 15, seizure 208 +- 33; n = 5 mice per group, t (5.56) = 2.91, P = .028). (G) Coronal sections at bregma 2.09 mm showing TRAPed neurons in motor and insular cortices, and olfactory nucleus. Regions marked are ac, anterior commissure; ACC, anterior cingulate cortex; AI, agranular insular cortex; AO, anter