PA5-58478

antibody from Invitrogen Antibodies

Targeting: METTL7B ALDI, MGC17301

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-58478 - Provider product page
Provider: Invitrogen Antibodies
Product name: METTL7B Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: RVLRPGGVLF FWEHVAEPYG SWAFMWQQVF EPTWKHIGDG CCLTRETWKD LENAQFSEIQ MERQPPPLKW LPVGPHIMGK AV Highest antigen sequence identity to the following orthologs: Mouse - 83%, Rat - 80%.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.2 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

METTL7B protein structure - PA5-58478 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Overexpression of the METTL7B gene in HeLa cells: ( A ) RT-PCR data measuring METTL7B gene expression in HeLa cells treated with a METTL7B overexpression plasmid compared to control cells transfected with an empty expression vector. ( B ) S -methyl captopril measured 24 h following the addition of captopril (500 uM) to HeLa cells treated with METTL7B overexpression plasmid compared to control cells. ( C ) FLAG-tagged METTL7B expression is only observed in HeLa cells treated with the METTL7B overexpression plasmid and not in controls. Western blot using an anti-FLAG antibody confirmed the expression of METTL7B in HeLa cells. Full gel images are available in Supplementary Fig. 1 . All data is presented as the mean +- s.d. Individual data points are plotted from two separate experiments. Significance was determined using unpaired two-tailed t test. **** P < 0.0001.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Purified His-GST-METTL7B: (Lane 1) SDS-PAGE silver stain of a representative gel showing purified His-GST-METTL7B (A). Gel lanes were loaded with a 1 µg total protein as determined by A 280. (Lane 2) Anti-GST antibody western blot of purified His-GST-METTL7B (1 µg total protein). (Lane 3) Western blot using anti-METTL7B antibody of purified His-GST-METTL7B (A) using 0.1 µg total protein. Molecular weight markers are shown to the left. The lower molecular bands, marked by letters B and C, are fusion protein fragments containing the dual His-GST affinity tag.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 SDS-PAGE and western blot analysis of purified His-GST-METTL7B-D98A: (Lane 1) SDS-PAGE silver stain of a representative gel showing purified His-GST-METTL7B-D98A (A) (4.2 µg total protein) (Lane 2) Anti-GST antibody western blot of purified His-GST-METTL7B-D98A (3 µg total protein). (Lane 3) Anti-METTL7B antibody western blot of purified His-GST-METTL7B-D98A (4.3 µg total protein). Molecular weight markers are shown to the left. Upper bands, above 55 kDa, are co-purifying chaperone proteins as determined by proteomic analysis (data not shown). Lower molecular bands, marked by letters B and C, are fusion protein fragments containing the dual His-GST affinity tag.