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Immunoprecipitation
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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- Product number
- 39937 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ago1/2/3 Monoclonal Antibody (2A8)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2A8
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Ago1/2/3 antibody (mAb) (Clone 2A8) tested by immunoprecipitation. The Active Motif miRNA IP Kit was used on samples of HT1080 cells that had been transfected with a miR-122 or a non-targeting miRNA control for 8 hours. Following IP using the Ago1/2/3 antibody or negative control IgG, qRT-PCR was performed on the samples using primers for G6PC3 and SLC1A5, which are known targets of miR-122, and for GAPDH, a common housekeeping gene that is not known to be targeted by miR-122.
