Ab089519

antibody from Aladdin Scientific
Targeting: AGO2 CASC7, EIF2C2, hAGO2, LINC00980, Q10
Provider product page for Ab089519

Antibody data

Product number
Ab089519 - Provider product page
Provider
Aladdin Scientific
Product name
Recombinant Argonaute-2 Antibody
Antibody type
Recombinant
Description
Rabbit anti Human Argonaute-2 Antibody, Recombinant, could be used for WB, IF, ICC and so on.ApplicationWB: 1/500-1/1000IF/ICC: 1/50-1/200Protein FunctionRequired for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
Reactivity
Human
Host
Rabbit
Conjugate
Unconjugated
Antigen sequence
AA 381-396
Vial size
100_l,10_l,1ml,50_l
Concentration
0,5 mg/ml
Storage
Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
AGO2 protein structure - Ab089519 shown in red.