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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- NB100-55251 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-55251, RRID:AB_2146900
- Product name
- Rabbit Polyclonal MYST1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Loss of the methyl lysine effector protein PHF20 impacts the expression of genes regulated by the lysine acetyltransferase MOF.
Modulations of hMOF autoacetylation by SIRT1 regulate hMOF recruitment and activities on the chromatin.
MOF and H4 K16 acetylation play important roles in DNA damage repair by modulating recruitment of DNA damage repair protein Mdc1.
Badeaux AI, Yang Y, Cardenas K, Vemulapalli V, Chen K, Kusewitt D, Richie E, Li W, Bedford MT
The Journal of biological chemistry 2012 Jan 2;287(1):429-37
The Journal of biological chemistry 2012 Jan 2;287(1):429-37
Modulations of hMOF autoacetylation by SIRT1 regulate hMOF recruitment and activities on the chromatin.
Lu L, Li L, Lv X, Wu XS, Liu DP, Liang CC
Cell research 2011 Aug;21(8):1182-95
Cell research 2011 Aug;21(8):1182-95
MOF and H4 K16 acetylation play important roles in DNA damage repair by modulating recruitment of DNA damage repair protein Mdc1.
Li X, Corsa CA, Pan PW, Wu L, Ferguson D, Yu X, Min J, Dou Y
Molecular and cellular biology 2010 Nov;30(22):5335-47
Molecular and cellular biology 2010 Nov;30(22):5335-47
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MYST1 Antibody [NB100-55251] - Detection of Human MOF/MYST1 on HeLa whole cell lysate using NB100-55251. MOF/MYST1 was also immunoprecipitated by rabbit anti-MOF/MYST1 antibodies NB100-55252 and NB100-55253.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MYST1 Antibody [NB100-55251] - Whole cell lysate (50 ug) from NIH3T3 cells. Antibodies: Affinity purified rabbit anti-MOF/MYST1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MYST1 Antibody [NB100-55251] - Detection of human MOF/MYST1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MOF/MYST1 antibody NB100-55251 (lot NB100-55251-2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). MOF/MYST1 was also immunoprecipitated by a previous lot (lot NB100-55251-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MYST1 Antibody [NB100-55251] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiMOF/MYST1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
