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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- PA5-47826 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WFDC2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of HT-29 human colon adenocarcinoma cell line and LNCaP human prostate cancer cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-human HE4/WFDC2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47826) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for HE4/WFDC2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA of WFDC2 in recombinant Human HE4/WFDC2 protein. Samples were incubated in WFDC2 polyclonal antibody (Product # PA5-47826). Protein was serially diluted 2-fold. Antibody was biotinylated and incubated with the protein captured on the clear polystyrene microplate. Detection of the standard curve was achieved by incubating Streptavidin-HRP followed by Substrate Solution and stopping the enzymatic reaction with Stop Solution.
