MAB7496

antibody from R&D Systems

Targeting: SPINK1 PCTT, PSTI, Spink3, TATI

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB7496

Provider

R&D Systems

Product name

Human SPINK1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human SPINK1 in ELISAs.

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P00995


Isotype

IgG

Antibody clone number

839305

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human SPINK1 by Western Blot. Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SPINK1 Monoclonal Antibody (Catalog # MAB7496) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SPINK1 at approximately 6 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human SPINK1 by Simple Western^TM. Simple Western lane view shows lysates of human pancreas tissue, loaded at 0.5 mg/mL. A specific band was detected for SPINK1 at approximately 6 kDa (as indicated) using 100 µg/mL of Mouse Anti-Human SPINK1 Monoclonal Antibody (Catalog # MAB7496). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.