LS-C313296

antibody from LSBio

Targeting: LPXN LDPL

Western blot (Data presented on provider website)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C313296

Provider

LSBio

Product name

LPXN / Leupaxin Antibody (aa115-129) LS-C313296

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

LPXN / Leupaxin antibody. IHC(P): Rat Spleen Tissue.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

LPXN / Leupaxin antibody. IHC(P): Mouse Spleen Tissue.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC analysis of Leupaxin using anti-Leupaxin antibody. Leupaxin was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Leupaxin antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.