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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- A303-814A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPP2R5C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PPP2R5C by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg), HeLa (H; 50 µg) and Jurkat (J; 50 µg) cells. Antibody: Affinity purified rabbit anti-PPP2R5C antibody (Product # A303-814A; lot A303-814A-1) used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PPP2R5C by western blot and immunoprecipitation. Samples: Whole cell lysate from 293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PPP2R5C antibody A303-814A used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. PPP2R5C was also immunoprecipitated by rabbit anti-PPP2R5C antibody A303-813A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PPP2R5C by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PPP2R5C (Cat. No. A303-814A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PPP2R5C by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PPP2R5C antibody (Product # A303-814A; lot A303-814A-1) used for IP at 6 µg/mg lysate. PPP2R5C was also immunoprecipitated by rabbit anti-PPP2R5C antibody (Product # A303-813A), which recognizes an upstream epitope. For blotting immunoprecipitated PPP2R5C (Product # A303-814A) was used at 1.0 µg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.
