MA1-16605

antibody from Invitrogen Antibodies

Targeting: PPP2R5C B56G, B56gamma, PR61G

Western blot (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-16605

Provider

Invitrogen Antibodies

Product name

Anti-PPP2R5C Monoclonal Antibody (TQ11-1G6)

Antibody type

Monoclonal

Antigen

Other

Description

Suggested positive control: HeLa nuclear extract.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

TQ11-1G6

Vial size

100 µL

Concentration

2 mg/ml

Storage

-20° C, Avoid Freeze/Thaw Cycles

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin immunoprecipitation analysis of PP2A B56 gamma-3 was performed using cross-linked chromatin from 1x10^6 HCT116 colon carcinoma cells treated with serum for 0, 15, 30, and 60 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0ul/100ul well volume of a PP2A B56 gamma-3 monoclonal antibody (Product # MA1-16605). Chromatin aliquots from ~1 x 105 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify exon 3-3 of human Bcl-x (hBcl-x) or exon 1 of human NR4A3 (hNR4A3). PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. Schematic representations of the Bcl-x and NR4A3 loci are shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions). Regions amplified by Bcl-x and NR4A3 primers are represented by black bars. Data courtesy of the Innovators Program.