Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C772963 - Provider product page
- Provider
- LSBio
- Product name
- DENND3 Antibody (phospho-Thr450) LS-C772963
- Antibody type
- Polyclonal
- Description
- Affinity purified from pooled serum
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of HeLa cell lysate showing specific immunolabeling of the ~142 kDa DENND3 protein phosphorylated at Thr450 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (?-Ptase, 800 units/1mg protein for 30 min).