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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB3649 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB3649, RRID:AB_10681230
- Product name
- SOS1 monoclonal antibody, clone SOS-01
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against synthetic peptide of SOS1.
- Antigen sequence
THPSMHRDGPPLLENAHSS- Isotype
- IgG
- Antibody clone number
- SOS-01
- Storage
- Store at 4°C. Do not freeze.Aliquot to avoid repeated freezing and thawing.
Submitted references Phosphoinositide lipid phosphatases: natural regulators of phosphoinositide 3-kinase signaling in T lymphocytes.
T cell-specific deletion of the inositol phosphatase SHIP reveals its role in regulating Th1/Th2 and cytotoxic responses.
Effects of SHIP-1 on MMP2 secretion and invasion of SR3Y1 cells.
Harris SJ, Parry RV, Westwick J, Ward SG
The Journal of biological chemistry 2008 Feb 1;283(5):2465-9
The Journal of biological chemistry 2008 Feb 1;283(5):2465-9
T cell-specific deletion of the inositol phosphatase SHIP reveals its role in regulating Th1/Th2 and cytotoxic responses.
Tarasenko T, Kole HK, Chi AW, Mentink-Kane MM, Wynn TA, Bolland S
Proceedings of the National Academy of Sciences of the United States of America 2007 Jul 3;104(27):11382-7
Proceedings of the National Academy of Sciences of the United States of America 2007 Jul 3;104(27):11382-7
Effects of SHIP-1 on MMP2 secretion and invasion of SR3Y1 cells.
Xing W, Hamaguchi M
Journal of genetics and genomics = Yi chuan xue bao 2007 Apr;34(4):285-93
Journal of genetics and genomics = Yi chuan xue bao 2007 Apr;34(4):285-93
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blotting analysis (reducing conditions) of human SOS1 using SOS1 monoclonal antibody, clone SOS-01 (Cat # MAB3649).Lane 1 : K-562 (human caucasian chronic myeloid leukemia cell line).Lane 2 : RAJI (human Burkitt lymphoma cell line).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Fig. A : Immunofluorescence staining of SOS1 in human primary fibroblasts using SOS1 monoclonal antibody, clone SOS-01 (Cat # MAB3649 ; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).Fig. B : Immunofluorescence staining of SOS1 in murine transformed fibroblasts using SOS1 monoclonal antibody, clone SOS-01 (Cat # MAB3649 ; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).
- Validation comment
- Immunofluorescence
