Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-78174 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: 293T, A431, HeLa, HepG2
- Concentration
- 2.63 mg/mL
Submitted references miR-152-3p Sensitizes Glioblastoma Cells Towards Cisplatin Via Regulation Of SOS1.
Wang M, Wu Q, Fang M, Huang W, Zhu H
OncoTargets and therapy 2019;12:9513-9525
OncoTargets and therapy 2019;12:9513-9525
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SOS1 in whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with SOS1 polyclonal antibody (Product # PA5-78174) using a dilution of 1:1000 followed by HRP-conjugated anti-rabbit IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SOS1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SOS1 Polyclonal Antibody (Product # PA5-78174) diluted at 1:500. Blue: Hoechst 33342 staining.
