- NBP2-42225 - Novus Biologicals CD63 antibody

Submitted references Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma.

Wang J, Wuethrich A, Sina AA, Lane RE, Lin LL, Wang Y, Cebon J, Behren A, Trau M
Science advances 2020 Feb;6(9):eaax3223

RNA cargos in extracellular vesicles derived from blood serum in pancreas associated conditions.

Kumar SR, Kimchi ET, Manjunath Y, Gajagowni S, Stuckel AJ, Kaifi JT
Scientific reports 2020 Feb 18;10(1):2800

Simvastatin Attenuates Cardiac Fibrosis via Regulation of Cardiomyocyte-Derived Exosome Secretion.

Kuo HF, Hsieh CC, Wang SC, Chang CY, Hung CH, Kuo PL, Liu YR, Li CY, Liu PL
Journal of clinical medicine 2019 Jun 4;8(6)

Pigment epithelium-derived factor inhibits lung cancer migration and invasion by upregulating exosomal thrombospondin 1.

Huang WT, Chong IW, Chen HL, Li CY, Hsieh CC, Kuo HF, Chang CY, Chen YH, Liu YP, Lu CY, Liu YR, Liu PL
Cancer letters 2019 Feb 1;442:287-298

Adenovirus early region 3 RIDα protein limits NFκB signaling through stress-activated EGF receptors.

Zeng X, Carlin CR
PLoS pathogens 2019 Aug;15(8):e1008017

The Susceptibilities of Respiratory Syncytial Virus to Nucleolin Receptor Blocking and Antibody Neutralization are Dependent upon the Method of Virus Purification.

Bilawchuk LM, Griffiths CD, Jensen LD, Elawar F, Marchant DJ
Viruses 2017 Aug 3;9(8)

Cooperative role for tetraspanins in adhesin-mediated attachment of bacterial species to human epithelial cells.

Green LR, Monk PN, Partridge LJ, Morris P, Gorringe AR, Read RC
Infection and immunity 2011 Jun;79(6):2241-9

Distinct roles for tetraspanins CD9, CD63 and CD81 in the formation of multinucleated giant cells.

Parthasarathy V, Martin F, Higginbottom A, Murray H, Moseley GW, Read RC, Mal G, Hulme R, Monk PN, Partridge LJ
Immunology 2009 Jun;127(2):237-48

Deficiency of niemann-pick type C-1 protein impairs release of human immunodeficiency virus type 1 and results in Gag accumulation in late endosomal/lysosomal compartments.

Tang Y, Leao IC, Coleman EM, Broughton RS, Hildreth JE
Journal of virology 2009 Aug;83(16):7982-95

Mapping of tetraspanin-enriched microdomains that can function as gateways for HIV-1.

Nydegger S, Khurana S, Krementsov DN, Foti M, Thali M
The Journal of cell biology 2006 Jun 5;173(5):795-807

Evidence that HIV budding in primary macrophages occurs through the exosome release pathway.

Nguyen DG, Booth A, Gould SJ, Hildreth JE
The Journal of biological chemistry 2003 Dec 26;278(52):52347-54

CD9 participates in endothelial cell migration during in vitro wound repair.

Klein-Soyer C, Azorsa DO, Cazenave JP, Lanza F
Arteriosclerosis, thrombosis, and vascular biology 2000 Feb;20(2):360-9

A general approach to the generation of monoclonal antibodies against members of the tetraspanin superfamily using recombinant GST fusion proteins.

Azorsa DO, Moog S, Cazenave JP, Lanza F
Journal of immunological methods 1999 Oct 29;229(1-2):35-48

Syncytium-inhibiting monoclonal antibodies produced against human T-cell lymphotropic virus type 1-infected cells recognize class II major histocompatibility complex molecules and block by protein crowding.

Hildreth JE
Journal of virology 1998 Dec;72(12):9544-52

CD63/Pltgp40: a platelet activation antigen identical to the stage-specific, melanoma-associated antigen ME491.

Azorsa DO, Hyman JA, Hildreth JE
Blood 1991 Jul 15;78(2):280-4

Characterization of a novel self-associating Mr 40,000 platelet glycoprotein.

Hildreth JE, Derr D, Azorsa DO
Blood 1991 Jan 1;77(1):121-32

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: CD63 Antibody (H5C6) [NBP2-42225] - THP1 whole cell protein was separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with anti-CD63 antibody at 2 ug/ml and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: CD63 Antibody (H5C6) [NBP2-42225] - The same samples and volumes were run under reducing and non-reducing conditions. All procedures were performed in parallel for both conditions. L- ladder +- exosomes. Image visualized with HRP linked to secondary antibody. This image was submitted via customer Review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - Human peripheral blood cells were stained (2 x 10^6 cells/ml) using the anti-CD63 antibody (Blue) at a dilution of 1:1000. Signal was detected using a Gt x Ms DyLight 488 Secondary and gated to the monocyte/granulocyte cell populations. Isotype was Mouse IgG1 kappa (orange). Data collected on BD FACS Calibur flow cytometer.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - A431 cells were stained (1 x10^6 cells/ml) using the anti-CD63 antibody at a 1:1000 dilution (blue). Signal was detected with Gt x Ms Dylight 488 secondary. Isotype control (orange).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow (Intracellular): CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on HepG2 cells with CD63 Antibody (H5C6) NBP2-42225AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on HeLa cells with CD63 Antibody (H5C6) NBP2-42225APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on SK-MEL-28 cells with CD63 Antibody (H5C6) NBP2-42225AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on SK-MEL-28 cells with CD63 (H5C6) Antibody NBP2-42225R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on HeLa cells with CD63 [H5C6] Antibody NBP2-42225AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

NBP2-42225

Antibody data