IC5048P-025

antibody from R&D Systems

Targeting: CD63 ME491, MLA1, TSPAN30

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC5048P-025

Provider

R&D Systems

Product name

Human CD63 PE-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Stains human CD63 transfectants but not irrelevant transfectants.

Reactivity

Human

Host

Mouse

Antigen sequence

P08962


Isotype

IgG

Antibody clone number

460305

Vial size

25 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of CD63 in NS0 Mouse Cell Line Transfected with Human CD63 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with Human CD63 and eGFP was stained with Mouse Anti-Human CD63 PE-conjugated Monoclonal Antibody (Catalog # IC5048P). Quadrant markers were set based on control antibody staining (Catalog # MAB002). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of CD63 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD63 PE-conjugated Monoclonal Antibody (Catalog # IC5048P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.