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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- V2070 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CD63 Antibody / LAMP-3
- Antibody type
- Monoclonal
- Description
- This highly specific CD63 antibody is suitable for use in Flow cytometry/Immunofluorescence/Immunohistochemistry applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- NKI/C3
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the CD63 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human spleen lysate with CD63 antibody (clone NKI/C3).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescence testing of HeLa cells with Alexa Fluor 488 conjugated CD63 antibody (green). F-actin filaments are labeled with Dylight 554 phalloidin (red); nuclei stained with DAPI (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human melanoma stained with CD63 antibody (NKI/C3).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of mouse spleen stained with CD63 antibody (NKI/C3).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of MCF-7 cells: Black=cells alone; Green=isotype control; Red=CD63 antibody PE conjugate
