Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- F49127 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CD63 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 163-190 from the human protein was used as the immunogen for this CD63 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the CD63 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of CD63 antibody at 1:2000 dilution. Lane 1: A2058 lysate; 2: A375 lysate; 3: THP-1 lysate; 4: U-87 MG lysate; Predicted band size: 25-60 kDa depending on glycosylation level.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of CD63 antibody at 1:1000 dilution + human plasma lysate; Predicted band size: 25-60 kDa depending on glycosylation level.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of CD63 antibody at 1:1000 dilution. Lane 1: A2058 lysate; 2: MDA-MB-453 lysate; Predicted band size: 25-60 kDa depending on glycosylation level.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human spleen tissue lysate with CD63 antibody. Predicted molecular weight: 25-60 kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human melanoma tissue with CD63 antibody at 1:25 dilution. HIER: steam tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of fixed and permeabilized human HL-60 cells with CD63 antibody. Blue = isotype control, Green = CD63 antibody.