Antibody data
- Antibody Data
 - Antigen structure
 - References [1]
 - Comments [0]
 - Validations
 - Immunocytochemistry [3]
 - Flow cytometry [1]
 
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Validation data
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- Product number
 - SM3035P - Provider product page

 - Provider
 - OriGene
 - Product name
 - CD63 mouse monoclonal antibody, clone MEM-259, Purified
 - Antibody type
 - Monoclonal
 - Description
 - CD63 mouse monoclonal antibody, clone MEM-259, Purified
 - Host
 - Mouse
 - Conjugate
 - Unconjugated
 - Epitope
 - CD63
 - Isotype
 - IgG
 - Antibody clone number
 - MEM-259
 - Vial size
 - 100 µg
 - Concentration
 - 1.0 mg/ml
 
Submitted references		The small chemical vacuolin-1 inhibits Ca(2+)-dependent lysosomal exocytosis but not cell resealing.
				
		
	
			Cerny J, Feng Y, Yu A, Miyake K, Borgonovo B, Klumperman J, Meldolesi J, McNeil PL, Kirchhausen T
EMBO reports 2004 Sep;5(9):883-8
		EMBO reports 2004 Sep;5(9):883-8
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					Supportive validation
					
									
				
				- Submitted by
 - OriGene (provider)
 - Main image
 
- Experimental details
 - Immunofluorescence staining of human skin fibroblasts with anti-CD63 antibody (MEM-259; green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).
 - Validation comment
 - IF
 
- Submitted by
 - OriGene (provider)
 - Main image
 
- Experimental details
 - Immunofluorescence staining of CD63 in human primary fibroblasts using anti-CD63 antibody (MEM-259; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
 - Validation comment
 - IF
 
- Submitted by
 - OriGene (provider)
 - Main image
 
- Experimental details
 - Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63 antibody (MEM-259; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
 - Validation comment
 - IF
 
							
					Supportive validation
					
									
				
		- Submitted by
 - OriGene (provider)
 - Main image
 
- Experimental details
 - Flow cytometry analysis of peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, stained with anti-human CD63 (MEM-259) FITC.
 - Validation comment
 - FC