Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [2]
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Validation data
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- Product number
- MAB6493 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB6493, RRID:AB_10676275
- Product name
- CD63 monoclonal antibody, clone MEM-259 (PerCP)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against native CD63.
- Conjugate
- Red dye
- Isotype
- IgG
- Antibody clone number
- MEM-259
- Storage
- Store in the dark at 4°C. Do not freeze.Avoid prolonged exposure to light.Aliquot to avoid repeated freezing and thawing.
Submitted references Expression of tetraspan protein CD63 activates protein-tyrosine kinase (PTK) and enhances the PTK-induced inhibition of ROMK channels.
CD63 as a biomarker for predicting the clinical outcomes in adenocarcinoma of lung.
The small chemical vacuolin-1 inhibits Ca(2+)-dependent lysosomal exocytosis but not cell resealing.
Lin D, Kamsteeg EJ, Zhang Y, Jin Y, Sterling H, Yue P, Roos M, Duffield A, Spencer J, Caplan M, Wang WH
The Journal of biological chemistry 2008 Mar 21;283(12):7674-81
The Journal of biological chemistry 2008 Mar 21;283(12):7674-81
CD63 as a biomarker for predicting the clinical outcomes in adenocarcinoma of lung.
Kwon MS, Shin SH, Yim SH, Lee KY, Kang HM, Kim TM, Chung YJ
Lung cancer (Amsterdam, Netherlands) 2007 Jul;57(1):46-53
Lung cancer (Amsterdam, Netherlands) 2007 Jul;57(1):46-53
The small chemical vacuolin-1 inhibits Ca(2+)-dependent lysosomal exocytosis but not cell resealing.
Cerny J, Feng Y, Yu A, Miyake K, Borgonovo B, Klumperman J, Meldolesi J, McNeil PL, Kirchhausen T
EMBO reports 2004 Sep;5(9):883-8
EMBO reports 2004 Sep;5(9):883-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence staining of CD63 in human HeLa cell line using CD63 monoclonal antibody, clone MEM-259 (Cat # MAB0931, green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue) .
- Validation comment
- Immunofluorescence
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence staining of (A) human skin fibroblasts and (B) human primary fibroblasts with CD63 monoclonal antibody, clone MEM-259 (Cat # MAB0931, green) after co-incubation of living cells with human Transferrin-Dyomics 547 (red) ; cell nuclei stained with DAPI (blue) .
- Validation comment
- Immunofluorescence