Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [7]
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Validation data
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- Product number
- 12-0639-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD63 Monoclonal Antibody (H5C6), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This H5C6 monoclonal antibody reacts with human CD63, a type III member of the tetraspanin family of transmembrane proteins. CD63 is expressed intracellularly on lysosomes, endosomes, and granules of resting platelets and basophils. However, cell surface expression of CD63 can be detected on activated basophils and platelets, monocytes, macrophages, and granulocytes. This receptor is also expressed on endothelial cells, fibroblasts, and smooth muscle cells. Studies have demonstrated that CD63 associates with integrins (VLA-3 and VLA-6) and TIMP-1 to mediate the allergic response. Applications Reported: This H5C6 antibody has been reported for use in flow cytometric analysis. Applications Tested: This H5C6 antibody has been pre-titrated and tested by flow cytometric of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- H5C6
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Stem cell-derived exosomes from human exfoliated deciduous teeth promote angiogenesis in hyperglycemic-induced human umbilical vein endothelial cells.
Excess fatty acids induce pancreatic acinar cell pyroptosis through macrophage M1 polarization.
M2-like tumor-associated macrophages transmit exosomal miR-27b-3p and maintain glioblastoma stem-like cell properties.
Unsupervised Machine Learning-Based Clustering of Nanosized Fluorescent Extracellular Vesicles.
Enrichment of CD44 in Exosomes From Breast Cancer Cells Treated With Doxorubicin Promotes Chemoresistance.
Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells.
Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.
Ionizing Radiation Increases the Activity of Exosomal Secretory Pathway in MCF-7 Human Breast Cancer Cells: A Possible Way to Communicate Resistance against Radiotherapy.
Urinary exosomes as a novel biomarker for evaluation of α-lipoic acid's protective effect in early diabetic nephropathy.
Pathological manifestations in lymphatic filariasis correlate with lack of inhibitory properties of IgG4 antibodies on IgE-activated granulocytes.
MicroRNA-containing extracellular vesicles released from endothelial colony-forming cells modulate angiogenesis during ischaemic retinopathy.
Human basophils are a source of - and are differentially activated by - IL-31.
The regulatory effect of UL-16 binding protein-3 expression on the cytotoxicity of NK cells in cancer patients.
Microvesicles derived from human umbilical cord Wharton's jelly mesenchymal stem cells attenuate bladder tumor cell growth in vitro and in vivo.
Sunartvanichkul T, Arayapisit T, Sangkhamanee SS, Chaweewannakorn C, Iwasaki K, Klaihmon P, Sritanaudomchai H
Journal of applied oral science : revista FOB 2023;31:e20220427
Journal of applied oral science : revista FOB 2023;31:e20220427
Excess fatty acids induce pancreatic acinar cell pyroptosis through macrophage M1 polarization.
Xia W, Lu Z, Chen W, Zhou J, Zhao Y
BMC gastroenterology 2022 Feb 19;22(1):72
BMC gastroenterology 2022 Feb 19;22(1):72
M2-like tumor-associated macrophages transmit exosomal miR-27b-3p and maintain glioblastoma stem-like cell properties.
Zhao G, Ding L, Yu H, Wang W, Wang H, Hu Y, Qin L, Deng G, Xie B, Li G, Qi L
Cell death discovery 2022 Aug 4;8(1):350
Cell death discovery 2022 Aug 4;8(1):350
Unsupervised Machine Learning-Based Clustering of Nanosized Fluorescent Extracellular Vesicles.
Kuypers S, Smisdom N, Pintelon I, Timmermans JP, Ameloot M, Michiels L, Hendrix J, Hosseinkhani B
Small (Weinheim an der Bergstrasse, Germany) 2021 Feb;17(5):e2006786
Small (Weinheim an der Bergstrasse, Germany) 2021 Feb;17(5):e2006786
Enrichment of CD44 in Exosomes From Breast Cancer Cells Treated With Doxorubicin Promotes Chemoresistance.
Wang X, Cheng K, Zhang G, Jia Z, Yu Y, Guo J, Hua Y, Guo F, Li X, Zou W, Sun H, Dong J, Yang Z
Frontiers in oncology 2020;10:960
Frontiers in oncology 2020;10:960
Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells.
Cheng L, Zhang K, Qing Y, Li D, Cui M, Jin P, Xu T
Journal of ovarian research 2020 Jan 22;13(1):9
Journal of ovarian research 2020 Jan 22;13(1):9
Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.
Menon V, Thomas R, Elgueta C, Horl M, Osborn T, Hallett PJ, Bartos M, Isacson O, Pruszak J
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Ionizing Radiation Increases the Activity of Exosomal Secretory Pathway in MCF-7 Human Breast Cancer Cells: A Possible Way to Communicate Resistance against Radiotherapy.
Jabbari N, Nawaz M, Rezaie J
International journal of molecular sciences 2019 Jul 25;20(15)
International journal of molecular sciences 2019 Jul 25;20(15)
Urinary exosomes as a novel biomarker for evaluation of α-lipoic acid's protective effect in early diabetic nephropathy.
Sun H, Yao W, Tang Y, Zhuang W, Wu D, Huang S, Sheng H
Journal of clinical laboratory analysis 2017 Nov;31(6)
Journal of clinical laboratory analysis 2017 Nov;31(6)
Pathological manifestations in lymphatic filariasis correlate with lack of inhibitory properties of IgG4 antibodies on IgE-activated granulocytes.
Prodjinotho UF, von Horn C, Debrah AY, Batsa Debrah L, Albers A, Layland LE, Hoerauf A, Adjobimey T
PLoS neglected tropical diseases 2017 Jul;11(7):e0005777
PLoS neglected tropical diseases 2017 Jul;11(7):e0005777
MicroRNA-containing extracellular vesicles released from endothelial colony-forming cells modulate angiogenesis during ischaemic retinopathy.
Dellett M, Brown ED, Guduric-Fuchs J, O'Connor A, Stitt AW, Medina RJ, Simpson DA
Journal of cellular and molecular medicine 2017 Dec;21(12):3405-3419
Journal of cellular and molecular medicine 2017 Dec;21(12):3405-3419
Human basophils are a source of - and are differentially activated by - IL-31.
Raap U, Gehring M, Kleiner S, Rüdrich U, Eiz-Vesper B, Haas H, Kapp A, Gibbs BF
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2017 Apr;47(4):499-508
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2017 Apr;47(4):499-508
The regulatory effect of UL-16 binding protein-3 expression on the cytotoxicity of NK cells in cancer patients.
Mou X, Zhou Y, Jiang P, Zhou T, Jiang Q, Xu C, Liu H, Zheng T, Yuan G, Zhang Y, Chen D, Mao C
Scientific reports 2014 Aug 20;4:6138
Scientific reports 2014 Aug 20;4:6138
Microvesicles derived from human umbilical cord Wharton's jelly mesenchymal stem cells attenuate bladder tumor cell growth in vitro and in vivo.
Wu S, Ju GQ, Du T, Zhu YJ, Liu GH
PloS one 2013;8(4):e61366
PloS one 2013;8(4):e61366
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD193 (CCR3) APC (Product # 17-1939-42), Anti-Human IgE FITC (Product # 11-6986-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD63 PE (purple histogram). CD193+IgE+ cells (left) were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 hWJMSC-MVs surface expressed molecules analysis. Flow cytometery analysis showed hWJMSC-MVs were positive for some surface expressed molecules typically expressed by MSCs, such as CD9, CD44, CD63, CD73, and negative for CD34, CD45.
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Characterization of endothelial colony-forming cells (ECFC)-derived extracellular vesicles (EVs). ( A ) Isolated ECFC-derived EVs were conjugated to CD63-coated latex beads to aid detection by flow cytometry. The gated bead-bound population (left panel) tested positive for EV markers CD9 and CD63. ( B ) Electron micrograph demonstrating the heterogeneity of the EV population (scale bar 1 mum). ( C ) Higher-magnification electron micrograph showing EVs of variable sizes and including a multivesicular body (scale bar 200 nm). ( D-F ) Electron micrographs of EVs bound to latex beads (LB) coated with anti-CD63 antibody. ( D ) White arrows indicate CD63-positive EVs of varying sizes (scale bar 1 mum). ( E ) Higher-magnification micrograph of EVs (scale bar 200 nm). In ( F ), a multivesicular body appears to be encapsulated within a CD63-positive membrane (scale bar 200 nm).
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 CTSS is produced in macrophage and transported by exosomes. Western blot analysis of CTSS in macrophage cell lysates and medium after PA (500 muM) stimulation for 24 h ( A ). Western blot analysis of exosome-specific proteins in exosomes and cell lysates detected 24 h after palmitic acid (PA, 500 muM) stimulation of macrophage ( B ). Electron microscopy of exosomes ( C ). (Scale bars = 500 nm) Exosome-derived CTSS from PA (500 muM) stimulated macrophage ( D ). QRT-PCR ( E ) and western blot ( F ) analysis of CTSS gene knockdown efficiency in macrophage. Data are shown as the mean +- SEM, n = 3, ANOVA. * P < 0.05. Detection of CTSS in exosomes from PA (500 muM) stimulated macrophage; macrophage were transfected with shCTSS, scr transfection as the control ( G )
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 M2-TAM-derived exosomes enhance the stemness of GSCs. A TEM images of M2-TAM-derived exosomes. The presence of CD63, CD206, and CD163 in M2-TAM-derived exosomes analyzed by flow cytometric ( B ) and Western blot ( C ) analyses. D Fluorescence images of GSCs co-cultured with Dil-labeled M2-TAM-derived exosomes with nuclei stained with DAPI (blue). E Representative images showing neurosphere formation of GSCs along with the statistics of GSC formation rate and diameter of spheres. F Expression of stem cell-related protein CD133, Nestin, Oct4, Sox2, and GFAP in GSCs measured by Western blot analysis. G HE staining images of xenograft tumors from mice. H The representative immunohistochemical images of Sox2 in xenograft tumors and the percentage of GSCs labeled by Sox2. I Kaplan-Meier survive curve of tumor-bearing mice. n = 10. * p < 0.05 vs. the control EXO group. Measurement data were depicted as mean +- standard deviation, comparison of that between two groups was conducted by unpaired t test. Cell experiments were repeated three times independently.
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 ( A ) Flow cytometric data confirmed exosomes by identifying exosomal marker CD63 and 90 +- 8% of exosomes in suspension represented CD63 marker. ( B ) Representative micrograph from transmission electron microscopy showing the nano-sized exosomes (Scale bar 40 nm). Exo: exosomes.
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Exosome isolation and analysis. A&C Representative transmission electron microscopy (TEM) images obtained for exosomes from HOSEPiC and SKOV-3. Scale bar: 100 nm. B&D flow cytometry analysis analyses show intensities for exosomal markers (CD9, CD63). (E) Particle size distribution by NanoSight analysis of exosomes from HOSEPiC and SKOV-3 (F) Western blot analyses show increased intensities for exosomal markers (Alix, TSG101)
- Conjugate
- Yellow dye