V8875

antibody from NSJ Bioreagents

Targeting: CD63 ME491, MLA1, TSPAN30

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V8875

Provider

NSJ Bioreagents

Product name

CD63 Antibody / LAMP-3

Antibody type

Monoclonal

Description

Purified

Reactivity

Human

Host

Mouse

Antibody clone number

LAMP3/4949

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Aliquot the CD63 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human tonsil tissue with CD63 antibody (clone LAMP3/4949) at 1ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS staining of bead-bound exosomes derived from MCF-7 cells: Unstained exosomes (gray) and exosomes stained with CD63 antibody (orange, clone LAMP3/4949).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS staining of human MCF-7 cells with CD63 antibody (blue, clone LAMP3/4949), unstained cells (blue, clone gray).

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free CD63 antibody (clone LAMP3/4949) as confirmation of integrity and purity.