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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [2]
- Other assay [1]
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- Product number
- V8875 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CD63 Antibody / LAMP-3
- Antibody type
- Monoclonal
- Description
- This highly specific CD63 antibody is suitable for use in ELISA/Flow cytometry/Immunofluorescence/Immunohistochemistry applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- LAMP3/4949
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Aliquot the CD63 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human tonsil tissue with CD63 antibody (clone LAMP3/4949) at 1ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS staining of bead-bound exosomes derived from MCF-7 cells: Unstained exosomes (gray) and exosomes stained with CD63 antibody (orange, clone LAMP3/4949).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS staining of human MCF-7 cells with CD63 antibody (blue, clone LAMP3/4949), unstained cells (blue, clone gray).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free CD63 antibody (clone LAMP3/4949) as confirmation of integrity and purity.
