Antibody data
- Antibody Data
 - Antigen structure
 - References [1]
 - Comments [0]
 - Validations
 - Western blot [1]
 - Immunocytochemistry [1]
 
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Validation data
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- Product number
 - AB0047-200 - Provider product page

 - Provider
 - SICGEN
 - Proper citation
 - SICGEN Cat#AB0047-200, RRID:AB_2333130
 - Product name
 - Anti-CD63
 - Antibody type
 - Polyclonal
 - Description
 - CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of the members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker.
 - Reactivity
 - Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
 - Host
 - Goat
 - Conjugate
 - Unconjugated
 - Isotype
 - IgG
 - Vial size
 - 600 µg
 - Concentration
 - 3 mg/ml
 - Storage
 - Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
 - Handling
 - The antibody solution should be gently mixed before use.
 
Submitted references		Presence of Cx43 in extracellular vesicles reduces the cardiotoxicity of the anti-tumour therapeutic approach with doxorubicin.
				
		
	
			Martins-Marques T, Pinho MJ, Zuzarte M, Oliveira C, Pereira P, Sluijter JP, Gomes C, Girao H
Journal of extracellular vesicles 2016;5:32538
		Journal of extracellular vesicles 2016;5:32538
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					Supportive validation
					
									
				
				- Submitted by
 - SICGEN (provider)
 - Main image
 
- Experimental details
 - Lysate at 50 µg per lane
 - Primary Ab dilution
 - 1/1,000
 - Conjugate
 - Unconjugated
 - Secondary Ab
 - Secondary Ab
 - Secondary Ab dilution
 - 1/10,000
 
							
					Supportive validation
					
									
				
		- Submitted by
 - SICGEN (provider)
 - Main image
 
- Experimental details
 - Cells were fixed with 4% PFA
 - Sample type
 - Hepa1-6
 - Primary Ab dilution
 - 1/50
 - Conjugate
 - Unconjugated