Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
Reference
Comment
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- Product number
- AB0047-500 - Provider product page
- Provider
- SICGEN
- Proper citation
- SICGEN Cat#AB0047-500, RRID:AB_2333131
- Product name
- Anti-CD63
- Antibody type
- Polyclonal
- Description
- Goat polyclonal to connexin 43, which is a member of the connexin family. Cx43 is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. This protein is the major protein of gap junctions in embryonic development and in the heart that are thought to have a crucial role in the synchronized contraction of the heart.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 1.5 mg
- Concentration
- 3 mg/ml
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Presence of Cx43 in extracellular vesicles reduces the cardiotoxicity of the anti-tumour therapeutic approach with doxorubicin.
Martins-Marques T, Pinho MJ, Zuzarte M, Oliveira C, Pereira P, Sluijter JP, Gomes C, Girao H
Journal of extracellular vesicles 2016;5:32538
Journal of extracellular vesicles 2016;5:32538
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Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Cell lysates at 50 µg per lane
- Sample type
- 3T3
- Primary Ab dilution
- 1/1,000
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1/10,000
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- cells were fixed with 4% of PFA
- Sample type
- Hepa1-6 cell line
- Primary Ab dilution
- 1/50
- Conjugate
- Unconjugated