14123-1-AP

antibody from Proteintech Group

Targeting: RPS3A MFTL, S3A

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

14123-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#14123-1-AP, RRID:AB_2253921

Product name

RPS3A antibody

Antibody type

Polyclonal

Description

KD/KO validated RPS3A antibody (Cat. #14123-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

The Rab5 effector FERRY links early endosomes with mRNA localization.

Schuhmacher JS, Tom Dieck S, Christoforidis S, Landerer C, Davila Gallesio J, Hersemann L, Seifert S, Schäfer R, Giner A, Toth-Petroczy A, Kalaidzidis Y, Bohnsack KE, Bohnsack MT, Schuman EM, Zerial M
Molecular cell 2023 Jun 1;83(11):1839-1855.e13

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Proteomic Landscape of Exosomes Reveals the Functional Contributions of CD151 in Triple-Negative Breast Cancer.

Li S, Li X, Yang S, Pi H, Li Z, Yao P, Zhang Q, Wang Q, Shen P, Li X, Ji J
Molecular & cellular proteomics : MCP 2021;20:100121

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Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons.

Cioni JM, Lin JQ, Holtermann AV, Koppers M, Jakobs MAH, Azizi A, Turner-Bridger B, Shigeoka T, Franze K, Harris WA, Holt CE
Cell 2019 Jan 10;176(1-2):56-72.e15

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On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons.

Shigeoka T, Koppers M, Wong HH, Lin JQ, Cagnetta R, Dwivedy A, de Freitas Nascimento J, van Tartwijk FW, Ströhl F, Cioni JM, Schaeffer J, Carrington M, Kaminski CF, Jung H, Harris WA, Holt CE
Cell reports 2019 Dec 10;29(11):3605-3619.e10

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RPS3A positively regulates the mitochondrial function of human periaortic adipose tissue and is associated with coronary artery diseases.

Tang Y, He Y, Li C, Mu W, Zou Y, Liu C, Qian S, Zhang F, Pan J, Wang Y, Huang H, Pan D, Yang P, Mei J, Zeng R, Tang QQ
Cell discovery 2018;4:52

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Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function.

Cioni JM, Wong HH, Bressan D, Kodama L, Harris WA, Holt CE
Neuron 2018 Mar 7;97(5):1078-1093.e6

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Proteomic profiling identified multiple short-lived members of the central proteome as the direct targets of the addicted oncogenes in cancer cells.

Qi T, Zhang W, Luan Y, Kong F, Xu D, Cheng G, Wang Y
Molecular & cellular proteomics : MCP 2014 Jan;13(1):49-62

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14123-1-AP(RPS3A antibody) at dilution of 1:800

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using RPS3A antibody 14123-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human pancreas cancer using 14123-1-AP(RPS3A antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human pancreas cancer using 14123-1-AP(RPS3A antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue