PA5-96463

antibody from Invitrogen Antibodies

Targeting: RPS3A MFTL, S3A

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96463

Provider

Invitrogen Antibodies

Product name

RPS3A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITED VQGKNCLTNF HGMDLTRDKM CSMVKKWQTM IEAHVDVKTT DGYLLRLFCV GFTKKRNNQI RKTSYAQHQQ VRQIRKKMME IMTREVQTND LKEVVNKLIP DSIGKDIEKA CQSIYPLHDV FVRKVKMLKK PKFELGKLME LHGEGSSSGK ATGDETGAKV ERADGYEPPV QESV; Positive Samples: MCF7, 293T, A-549, Mouse liver, Mouse lung, Mouse ovary, Mouse pancreas, Rat ovary; Cellular Location: Cytoplasm, Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.8 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of RPS3A was performed in MCF-7 cells using RPS3A Polyclonal Antibody (Product # PA5-96463).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of RPS3A in MCF-7 cells. Samples were incubated with RPS3A Polyclonal antibody (Product # PA5-96463). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of RPS3A in 200 μg extracts of MCF7 cells. Samples were precipitated with 3 μg RPS3A Polyclonal antibody (Product # PA5-96463). Western blot was performed from the immunoprecipitate using RPS3A Polyclonal antibody (Product # PA5-96463) at a dilution of 1:500.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPS3A in paraffin-embedded rat brain. Samples were incubated with RPS3A Polyclonal antibody (Product # PA5-96463) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPS3A in paraffin-embedded human liver cancer. Samples were incubated with RPS3A Polyclonal antibody (Product # PA5-96463) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPS3A in paraffin-embedded mouse brain. Samples were incubated with RPS3A Polyclonal antibody (Product # PA5-96463) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of RPS3A was performed in 200 µg extracts of MCF7 cells using RPS3A Polyclonal Antibody (Product # PA5-96463). Western blot was performed from the immunoprecipitate using RPS3A Polyclonal Antibody at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of RPS3A was performed in 200 µg extracts of MCF7 cells using RPS3A Polyclonal Antibody (Product # PA5-96463). Western blot was performed from the immunoprecipitate using RPS3A Polyclonal Antibody at a dilution of 1:500.