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Learn25045-1-AP
antibody from Invitrogen Antibodies
Targeting: LPP
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 25045-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LPP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSHPSWLPP KSTGEPLGHV PARMETTHSF GNPSISVSTQ QPPKKFAPVV APKPKYNPYK QPGGEGDFLP PPPPPLDDSS ALPSISGNFP PPPPLDEEAF KVQGNPGGKT LEERRSSLDA EIDSLTSILA DLECSSPYKP RPPQSSTGST ASPPVSTPVT GHKRMVIPNQ PPLTATKKST LKPQPAPQAG PIPVAPIGTL KPQPQPVPAS YTTASTSSRP TFNVQVKSAQ PSPHYMAAPS SGQIYGSGPQ GYNTQPVPVS GQCPPPSTRG GMDYAYIPPP GLQPEPGYGY APNQGRYYEG YYAAGPGYGG RNDSDPTYGQ QGHPNTWKRE PGYTPPGAGN QNPPGMYPVT G (1-350 aa encoded by BC130584)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse colon tissue were subjected to SDS PAGE followed by western blot with 25045-1-AP ( LPP Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 25045-1-AP (LPP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 25045-1-AP ( LPP Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 25045-1-AP ( LPP Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.05ug LPP antibody (25045-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-Goat anti-Rabbit IGG with dilution 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-LPP (IP:25045-1-AP, 4ug; Detection:25045-1-AP 1:1000) with HeLa cells lysate 3200ug.
