Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [6]
- Flow cytometry [1]
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- Product number
- MA5-32231 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Moesin Recombinant Rabbit Monoclonal Antibody (SC69-01)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SC69-01
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Moesin in different lysates using a Monoclonal antibody (Product #MA5-32231) at a dilution of 1:1,000. Positive control: Lane 1: Raji, Lane 2: SH-SY-5Y.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Moesin was performed by loading 25 µg of WT (lane 1) and MSN CRISPR KO (lane 2) HeLa cell lysates in RIPA buffer onto a 4-15% gradient polyacrylamide gel. Proteins were transferred to nitrocellulose membrane and blocked in 5% milk. Ponceau stained transfer of blot is shown. Moesin was detected at approximately 68 kDa using a Moesin recombinant monoclonal antibody (Product # MA5-32231) at a dilution of 1:1,000 in 5% BSA in TBST overnight at 4 deg, followed by secondary antibody diluted to 0.2 µg/mL using Goat anti-Rabbit IgG (H+L) HRP antibody (Product # 65-6120). Chemiluminescent detection was performed using Pierce ECL Western Blotting Substrate (Product # 32106). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Moesin in Hela cells using a Moesin Monoclonal antibody (Product # MA5-32231) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Moesin in NCCIT cells using a Moesin Monoclonal antibody (Product # MA5-32231) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of Moesin was performed using parental and MSN CRISPR KO cells that were transfected with a GFP or mCherry plasmid, respectively. At 48 hrs post transfection parental and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA for 15 min and permeabilized with 0.1% Triton X-100. Cells were stained with Moesin recombinant monoclonal antibody (Product # MA5-32231) at a 1:500 dilution overnight at 4 deg. Secondary antibody incubation was performed using 1 µg/mL of Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Alexa Fluor 647 antibody (Product # A-21245) for 1 hr at RT. Imaging was performed with a 40X oil objective and analysis was performed using Image J. Cell image represents a single focal plane. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Moesin of paraffin-embedded Human tonsil tissue using a Moesin Monoclonal antibody (Product #MA5-32231). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Moesin of paraffin-embedded Human spleen tissue using a Moesin Monoclonal antibody (Product #MA5-32231). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Moesin of paraffin-embedded Human breast carcinoma tissue using a Moesin Monoclonal antibody (Product #MA5-32231). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Moesin of paraffin-embedded Mouse lung tissue using a Moesin Monoclonal antibody (Product #MA5-32231). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Moesin of paraffin-embedded Mouse placenta tissue using a Moesin Monoclonal antibody (Product #MA5-32231). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Moesin of paraffin-embedded Mouse stomach tissue using a Moesin Monoclonal antibody (Product #MA5-32231). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of Moesin in Hela cells using a Moesin Monoclonal Antibody (Product # MA5-32231) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.