HPA011227

MSN antibody from Atlas Antibodies

Eligible for validation within the Antibodypedia Validation Initiative

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Validation data

Product number: HPA011227
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA011227, RRID:AB_1234871
Product name: Anti-MSN
Provider product page: Atlas Antibodies - HPA011227
Antibody type: Polyclonal
Description: Affinity purified using the PrEST antigen as affinity ligand
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: AELKTAMSTPHVAEPAENEQDEQDENGAEASADLR ADAMAKDRSEEERTTEAEKNERVQKHLKALTSELA NARDESKKTANDMIHAENMRLGRDKYKTLRQIRQG NTKQR
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Sample type: MOUSE, RAT

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Sample type: MOUSE, RAT

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Western blot analysis in human cell lines U-251MG and MCF-7 using Anti-MSN antibody. Corresponding MSN RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Western blot analysis using Anti-MSN antibody HPA011227 (A) shows similar pattern to independent antibody HPA011135 (B).
Sample type: HUMAN
Antibody #2 product nr: HPA011135
Antibody provider: Atlas Antibodies
Independent Antibody Method: WB