PA5-34744

antibody from Invitrogen Antibodies

Targeting: CMTM6 CKLFSF6, FLJ20396

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-34744 - Provider product page
Provider: Invitrogen Antibodies
Product name: CMTM6 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Recommended positive controls: 293T, HeLa, HepG2, Jurkat, Raji, NCI-H929, mouse placenta, rat testis. Predicted reactivity: Rhesus Monkey (100%), Chimpanzee (100%). IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.24 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CMTM6 protein structure - PA5-34744 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CMTM6 using 30 µg of HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a CMTM6 polyclonal antibody (Product # PA5-34744) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of CMTM6 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CMTM6 Polyclonal Antibody (Product # PA5-34744) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CMTM6 was performed by separating 50 µg of mouse tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CMTM6 Polyclonal Antibody (Product # PA5-34744) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CMTM6 was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CMTM6 Polyclonal Antibody (Product # PA5-34744) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using CMTM6 Polyclonal Antibody (Product # PA5-34744). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CMTM6 Polyclonal Antibody (Product # PA5-34744) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of CMTM6 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CMTM6 Polyclonal Antibody (Product # PA5-34744) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of CMTM6 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CMTM6 Polyclonal Antibody (Product # PA5-34744) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.