Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-36808 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-GRB10 (Tyr67) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 67 kDa.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-GRB10 pTyr67 using Phospho-GRB10 pTyr67 polyclonal antibody (Product # PA5-36808) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with insulin (100nM, 5min), Lane 2: Mouse spleen tissue lysate, Lane 3: PC12 cell lysate treated with insulin (100nM, 5min).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-GRB10 (Tyr67) in Lane 1: HEK293T cell lysate treated with insulin (100 nM,5mins), Lane 2: mouse spleen tissue lysate, Lane 3: PC12 cell lysate treated with insulin (100 nM,5mins). Samples were incubated with Phospho-GRB10 (Tyr67) polyclonal antibody (Product # PA5-36808) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-GRB10 (Tyr67) in paraffin-embedded human kidney carcinoma tissue (cytoplasmic staining) and negative control (right, with PBS only). Samples were incubated with Phospho-GRB10 (Tyr67) polyclonal antibody (Product # PA5-36808) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.