12134-2-AP

antibody from Invitrogen Antibodies

Targeting: UBE2C UBCH10

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12134-2-AP

Provider

Invitrogen Antibodies

Product name

UBE2C Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MASQNRDPA ATSVAAARKG AEPSGGAARG PVGKRLQQEL MTLMMSGDKG ISAFPESDNL FKWVGTIHGA AGTVYEDLRY KLSLEFPSGY PYNAPTVKFL TPCYHPNVDT QGNICLDILK EKWSALYDVR TILLSIQSLL GEPNIDSPLN THAAELWKNP TAFKKYLQET YSKQVTSQEP (1-179 aa encoded by BC016292)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12134-2-AP (UBE2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12134-2-AP (UBE2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 12134-2-AP ( UBE2C Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 12134-2-AP ( UBE2C Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-UBE2C (IP:12134-2-AP, 3ug; Detection:12134-2-AP 1:300) with HeLa cells lysate 2440ug.